The Anti-Biotin Antibody (CAB18848) offered by Assay Genie is a highly specific and sensitive antibody designed for research involving biotinylated molecules. This polyclonal antibody, raised in rabbits, is ideal for use in various immunological assays, such as ELISA, immunoprecipitation, and flow cytometry.The antibody binds specifically to biotin, allowing for accurate detection and quantification of biotinylated proteins in samples. Its high reactivity with biotin makes it a valuable tool for researchers studying protein biotinylation, protein-protein interactions, and signal transduction pathways involving biotinylated molecules.
With its versatility and sensitivity, the Anti-Biotin Antibody (CAB18848) is a valuable asset for studies in biochemistry, cell biology, and molecular biology. Its reliability and performance make it a trusted reagent for researchers looking to explore the role of biotin in various cellular processes and signaling pathways.
Product Name:
Biotin Rabbit Polyclonal Antibody
SKU:
CAB18848
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Species independent
Immunogen:
Biotin
Tested Applications:
WB
Recommended Dilution:
WB,1:500 - 1:2000
Positive Sample:
Biotin-Goat-Mouse IgG
Observed MW:
25kDa(Light chain),55kDa(Heavy chain)
It is well know now that biotin plays important roles in a variety of critical metabolic reactions in the cell, and thus, is essential for normal human health, growth and development. Biotin is a water-soluble vitamin required by all organisms by virtue of its essential role in carboxylation reactions. Streptavidin and its homologs (together referred to as streptavidin) are widely used in molecular science owing to their highly selective and stable interaction with biotin.
Purification Method:
Affinity purification
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of recombinant Biotin-Goat-Mouse IgG Protein using Biotin Rabbit pAb (CAB18848) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 100 ng per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 45s.
