The Anti-BCL2L12 Antibody (CAB19808) is a highly specific antibody designed for research involving BCL2L12, a gene that encodes a member of the Bcl-2 family of proteins. This antibody is raised in rabbits and is validated for use in various applications such as Western blot and immunohistochemistry. It specifically binds to the BCL2L12 protein, enabling accurate detection and analysis in human samples.BCL2L12 is a pro-apoptotic protein that plays a role in regulating cell survival and apoptosis. Dysregulation of BCL2L12 has been implicated in various diseases, including cancer.
Research into the function and regulation of BCL2L12 is important for understanding its role in disease pathogenesis and for developing targeted therapies.This antibody is a valuable tool for researchers in the fields of apoptosis, cancer biology, and molecular biology. Its high specificity and reactivity with human samples make it ideal for studying the function and regulation of BCL2L12 in a variety of cell types and tissues. By using this antibody, researchers can gain valuable insights into the role of BCL2L12 in disease and potentially identify new therapeutic targets.
Product Name:
BCL2L12 Rabbit Monoclonal Antibody
SKU:
CAB19808
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 200-300 of human BCL2L12 (Q9HB09).
WB,1:500 - 1:2000 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cellsIHC-P,1:50 - 1:200
Positive Sample:
MCF7,PC-3
Conjugate:
Unconjugated
Cellular Localization:
Nucleus
Calculated MW:
37kDa
Observed MW:
32kDa
This gene encodes a member of a family of proteins containing a Bcl-2 homology domain 2 (BH2). The encoded protein is an anti-apoptotic factor that acts as an inhibitor of caspases 3 and 7 in the cytoplasm. In the nucleus, it binds to the p53 tumor suppressor protein, preventing its association with target genes. Overexpression of this gene has been detected in a number of different cancers. There is a pseudogene for this gene on chromosome 3. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
83596
Clone Number:
ARC2334
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using BCL2L12 Rabbit mAb (CAB19808) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
