BCKDK Monoclonal Antibody (CAB20909)
- SKU:
- CAB20909
- Product Type:
- Antibody
- Antibody Type:
- Polyclonal Antibody
- Reactivity:
- Plant
- Host Species:
- Rabbit
- Isotype:
- IgG
Frequently bought together:
Description
| Product Name: | BCKDK Monoclonal Antibody |
| SKU: | CAB20909 |
| Size: | 20uL, 100uL |
| Isotype: | IgG |
| Host Species: | Rabbit |
| Reactivity: | Human,Mouse,Rat |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human BCKDK (O14874). |
| Sequence: | MILA SVLR SGPG GGLP LRPL LGPA LALR ARST SATD THHV EMAR ERSK TVTS FYNQ SAID AAAE KPSV RLTP TMML YAGR SQDG SHLL KSAR YLQQ ELPV |
| Tested Applications: | WB ELISA |
| Recommended Dilution: | WB,1:100 - 1:500 |
| Synonyms: | BDK; BCKDKD; BCKDK |
| Positive Sample: | HeLa,HepG2,PC-12,Mouse pancreas |
| Conjugate: | Unconjugated |
| Cellular Localization: | Mitochondrion, Mitochondrion matrix. |
| Calculated MW: | 46kDa |
| Observed MW: | 46kDa |
The branched-chain alpha-ketoacid dehydrogenase complex (BCKD) is an important regulator of the valine, leucine, and isoleucine catabolic pathways. The protein encoded by this gene is found in the mitochondrion, where it phosphorylates and inactivates BCKD. Several transcript variants encoding different isoforms have been found for this gene.
| Purification Method: | Affinity purification |
| Gene ID: | 10295 |
| Clone Number: | ARC2875 |
| Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3. |
 | Western blot analysis of extracts of various cell lines, using BCKDK antibody (CAB20909) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s. |
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