The BCKDK Polyclonal Antibody (CAB20909) is a valuable tool for researchers studying BCKDK, an enzyme involved in branched-chain amino acid metabolism. This antibody is produced in rabbits and has high reactivity with human samples, making it suitable for use in Western blot applications. By binding to the BCKDK protein, this antibody enables the detection and analysis of BCKDK in various cell types, offering researchers valuable insights into metabolic processes and potential therapeutic targets.
BCKDK, or branched-chain ketoacid dehydrogenase kinase, plays a crucial role in the regulation of branched-chain amino acid metabolism, a pathway linked to various metabolic disorders and diseases. Understanding the function of BCKDK is essential for uncovering the molecular mechanisms underlying these conditions and developing targeted interventions. The BCKDK Polyclonal Antibody is a reliable tool for investigating BCKDK function and its implications in metabolic health and disease.
Product Name:
BCKDK Monoclonal Antibody
SKU:
CAB20909
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human BCKDK (O14874).
Sequence:
MILA SVLR SGPG GGLP LRPL LGPA LALR ARST SATD THHV EMAR ERSK TVTS FYNQ SAID AAAE KPSV RLTP TMML YAGR SQDG SHLL KSAR YLQQ ELPV
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:100 - 1:500
Synonyms:
BDK; BCKDKD; BCKDK
Positive Sample:
HeLa,HepG2,PC-12,Mouse pancreas
Conjugate:
Unconjugated
Cellular Localization:
Mitochondrion, Mitochondrion matrix.
Calculated MW:
46kDa
Observed MW:
46kDa
The branched-chain alpha-ketoacid dehydrogenase complex (BCKD) is an important regulator of the valine, leucine, and isoleucine catabolic pathways. The protein encoded by this gene is found in the mitochondrion, where it phosphorylates and inactivates BCKD. Several transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
10295
Clone Number:
ARC2875
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using BCKDK antibody (CAB20909) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
