[KO Validated] Bax Rabbit Monoclonal Antibody (CAB20227)
- SKU:
- CAB20227
- Product Type:
- Antibody
- Antibody Type:
- Monoclonal Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Synonyms:
- BCL2L4
- BAX
- Research Area:
- Cell Death
Description
| Product Name: | [KO Validated] Bax Rabbit Monoclonal Antibody |
| SKU: | CAB20227 |
| Size: | 20uL, 100uL |
| Isotype: | IgG |
| Host Species: | Rabbit |
| Reactivity: | Human,Mouse,Rat |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 10-70 of human Bax (NP_620116.1). |
| Sequence: | GGGP TSSE QIMK TGAL LLQG FIQD RAGR MGGE APEL ALDP VPQD ASTK KLSE CLKR IGDE L |
| Tested Applications: | WB IF/ICC IP ELISA |
| Recommended Dilution: | WB,1:2000 - 1:10000 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells |
| Synonyms: | BCL2L4; ax |
| Positive Sample: | 293T,HT-1080,RAW264.7 |
| Conjugate: | Unconjugated |
| Cellular Localization: | Cytoplasm, Cytoplasm, Mitochondrion membrane, Single-pass membrane protein. |
| Calculated MW: | 21kDa |
| Observed MW: | 21kDa |
The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. The association and the ratio of BAX to BCL2 also determines survival or death of a cell following an apoptotic stimulus. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene.
| Purification Method: | Affinity purification |
| Gene ID: | 581 |
| Clone Number: | ARC5006-10 |
| Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3. |
 | Western blot analysis of various lysates, using [KO Validated] Bax Rabbit mAb (CAB20227) at 1:10000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s. |
Related Products
[KO Validated] Bax Rabbit Polyclonal Antibody (CAB12009)
[KO Validated] Bax Rabbit Polyclonal Antibody (CAB0207)
[KO Validated] CD47 Rabbit Monoclonal Antibody (CAB11382)
[KO Validated] MTA2 Rabbit Monoclonal Antibody (CAB4624)
