The Anti-Bax KO Validated Antibody (CAB20227) is a essential tool for researchers studying the Bax protein, a key regulator of apoptosis, or programmed cell death. This antibody, validated for use in knockout (KO) samples, is raised in rabbits and exhibits high reactivity with human samples. It is optimized for use in Western blot applications, allowing for the precise detection and analysis of the Bax protein in various cell types.Bax is a critical component of the intrinsic apoptosis pathway, playing a central role in controlling cell death processes and maintaining cellular homeostasis. Dysregulation of Bax expression has been implicated in a wide range of diseases, including cancer, neurodegenerative disorders, and cardiovascular conditions.
By targeting Bax, researchers can uncover new insights into the mechanisms of cell death and potentially develop novel therapies for treating these diseases.The Anti-Bax KO Validated Antibody is an invaluable tool for investigating the function and regulation of the Bax protein in various biological contexts. Its specificity and reliability make it a trusted reagent for researchers in the fields of cell biology, oncology, and molecular medicine. Using this antibody, scientists can advance their understanding of apoptosis and potentially discover new avenues for therapeutic intervention.
Product Name:
[KO Validated] Bax Rabbit Monoclonal Antibody
SKU:
CAB20227
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 10-70 of human Bax (NP_620116.1).
The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. The association and the ratio of BAX to BCL2 also determines survival or death of a cell following an apoptotic stimulus. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene.
Purification Method:
Affinity purification
Gene ID:
581
Clone Number:
ARC5006-10
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates, using [KO Validated] Bax Rabbit mAb (CAB20227) at 1:10000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
