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ATP6V1G2 Rabbit Polyclonal Antibody (CAB20163)

SKU:
CAB20163
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Synonyms:
NG38
ATP6G
VMA10
ATP6G2
$167 - $503
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:ATP6V1G2 Rabbit Polyclonal Antibody
SKU:CAB20163
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 40-100 of human ATP6V1G2 (NP_569730.1).
Sequence:AQME VEQY RRER EHEF QSKQ QAAM GSQG NLSA EVEQ ATRR QVQG MQSS QQRN RERV LAQL L
Tested Applications:WB IHC-P ELISA
Recommended Dilution:WB,1:500 - 1:1000 IHC-P,1:50 - 1:200
Synonyms:NG38; ATP6G; VMA10; ATP6G2; ATP6V1G2
Positive Sample:SH-SY5Y,Mouse brain,Rat brain,Mouse eye
Conjugate:Unconjugated
Cellular Localization:cytosol, extrinsic component of synaptic vesicle membrane
Calculated MW:14kDa
Observed MW:14kDa

This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of intracellular compartments of eukaryotic cells. V-ATPase dependent acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c'', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is one of three V1 domain G subunit proteins. This gene had previous gene symbols of ATP6G and ATP6G2. Alternatively spliced transcript variants encoding different isoforms have been described. Read-through transcription also exists between this gene and the downstream DEAD (Asp-Glu-Ala-Asp) box polypeptide 39B (DDX39B) gene.

Purification Method:Affinity purification
Gene ID:534
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using ATP6V1G2 Rabbit pAb (CAB20163) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.

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