The Anti-ATP4B Antibody (CAB19280) is a highly specific tool for researchers studying ATP4B, a protein involved in gastric acid secretion and maintenance of the gastric mucosa. This polyclonal antibody, produced in rabbits, exhibits strong reactivity with human samples and has been validated for use in applications such as Western blotting.ATP4B, also known as H+/K+ transporting ATPase subunit beta, is essential for the functioning of the proton pump in parietal cells of the stomach. Dysregulation of ATP4B has been implicated in various gastric disorders, including gastric ulcers and gastritis.
By targeting ATP4B with this antibody, researchers can explore the role of this protein in gastric physiology and pathology.This antibody provides a valuable tool for investigating the mechanisms underlying gastric acid secretion and the potential therapeutic implications for disorders related to ATP4B dysfunction. Its high specificity and reactivity make it an ideal choice for researchers in the fields of gastroenterology and cell biology.
Product Name:
ATP4B Rabbit Monoclonal Antibody
SKU:
CAB19280
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 192-291 of human ATP4B (P51164).
Cell membrane, Single-pass type II membrane protein.
Calculated MW:
33kDa
Observed MW:
50kDa
The protein encoded by this gene belongs to a family of P-type cation-transporting ATPases. The gastric H+, K+-ATPase is a heterodimer consisting of a high molecular weight catalytic alpha subunit and a smaller but heavily glycosylated beta subunit. This enzyme is a proton pump that catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. It is also responsible for gastric acid secretion. This gene encodes the beta subunit of the gastric H+, K+-ATPase.
Purification Method:
Affinity purification
Gene ID:
496
Clone Number:
ARC2461
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using ATP4B Rabbit mAb (CAB19280) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
