Asymmetric DiMethyl-Histone H3-R2 Polyclonal Antibody (CAB20732)
- SKU:
- CAB20732
- Product Type:
- Antibody
- Antibody Type:
- Secondary Antibody
- Host Species:
- Goat
- Isotype:
- Horseradish peroxidase conjugated IgG
Description
| Product Name: | Asymmetric DiMethyl-Histone H3-R2 Polyclonal Antibody |
| SKU: | CAB20732 |
| Size: | 20uL, 100uL |
| Isotype: | IgG |
| Host Species: | Rabbit |
| Reactivity: | Human,Mouse,Rat,Other (Wide Range Predicted) |
| Immunogen: | A synthetic asymmetric dimethylated peptide around R2 of human Histone H3 (NP_003520.1). |
| Sequence: | MART |
| Tested Applications: | WB ELISA |
| Recommended Dilution: | WB,1:500 - 1:1000 |
| Synonyms: | H3/A; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3FA; H3C10; H3C11; H3C12; HIST1H3A; Asymmetric DiMethyl-Histone H3-R2 |
| Positive Sample: | HeLa acid extract,NIH/3T3 acid extract,C6 acid extract,H3 protein |
| Conjugate: | Unconjugated |
| Cellular Localization: | Chromosome, Nucleus. |
| Calculated MW: | 15kDa |
| Observed MW: | 17kDa |
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.
| Purification Method: | Affinity purification |
| Gene ID: | 8290/8350 |
| Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3. |
 | Western blot analysis of various lysates using Asymmetric DiMethyl-Histone H3-R2 Rabbit pAb (CAB20732) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 90s. |
Related Products
DiMethyl-Histone H3-K79 Polyclonal Antibody (CAB20822)
