The Anti-ARF12 Antibody (CAB20635) from Assay Genie is a high-quality polyclonal antibody specifically designed for research involving ARF12, a member of the ADP-ribosylation factor family. This antibody, raised in rabbits, is highly reactive with human samples and has been validated for use in applications such as Western blot, immunofluorescence, and immunohistochemistry.ARF12 is a small GTPase protein that plays a crucial role in regulating intracellular vesicle trafficking and membrane dynamics. It is involved in processes such as endocytosis, exocytosis, and cytoskeletal organization, making it a key player in cellular homeostasis and signaling.
The Anti-ARF12 Antibody binds specifically to ARF12, allowing for the detection and analysis of this protein in various cell types.Research on ARF12 is essential for understanding its functions in cellular processes and its potential implications in diseases such as cancer, neurodegenerative disorders, and infectious diseases. By using the Anti-ARF12 Antibody, researchers can shed light on the role of ARF12 in these contexts and potentially identify novel therapeutic targets for intervention.
Product Name:
ARF12 Rabbit Polyclonal Antibody
SKU:
CAB20635
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Oryza sativa
Immunogen:
Recombinant protein of Oryza sativa ARF12.
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:2000
Synonyms:
ARF8; ARF12; OsARF8; OsJ_16583
Positive Sample:
spikes
Conjugate:
Unconjugated
Calculated MW:
91kDa
Observed MW:
100kDa
Auxin response factors (ARFs) are transcriptional factors that bind specifically to the DNA sequence 5'-TGTCTC-3' found in the auxin-responsive promoter elements (AuxREs).
Purification Method:
Affinity purification
Gene ID:
4337363
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of various tissues from the japonica rice (Oryza sativa L.) variety Zhonghua 11, using ARF12 Rabbit pAb (CAB20635) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3s.
