The Anti-AP2S1 Antibody (CAB9263) is a high-quality research tool designed for studies involving AP2S1, a protein involved in the clathrin-mediated endocytosis pathway. This polyclonal antibody, raised in rabbits, is specifically reactive with human samples and is validated for use in various applications, including Western blot and immunofluorescence.AP2S1 is a subunit of the AP2 adaptor protein complex, which plays a crucial role in the internalization of cell surface receptors and other membrane proteins. Dysregulation of this pathway has been linked to various diseases, including cancer and neurological disorders.
The Anti-AP2S1 Antibody enables precise detection and analysis of AP2S1 protein levels in different cell types, making it a valuable tool for researchers studying endocytosis and its implications in health and disease.By understanding the function and regulation of AP2S1, researchers can gain insights into cellular processes that are essential for normal physiological functions and disease development. The Anti-AP2S1 Antibody provides a reliable means to investigate AP2S1 expression and localization, facilitating research in molecular biology, cell biology, and pharmacology.
Product Name:
AP2S1 Rabbit Monoclonal Antibody
SKU:
CAB9263
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human AP2S1 (P53680).
clathrin-coated endocytic vesicle, cytoplasmic side of plasma membrane, cytosol, plasma membrane, postsynapse, presynapse
Calculated MW:
17kDa
Observed MW:
17kDa
One of two major clathrin-associated adaptor complexes, AP-2, is a heterotetramer which is associated with the plasma membrane. This complex is composed of two large chains, a medium chain, and a small chain. This gene encodes the small chain of this complex. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
1175
Clone Number:
ARC1500
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of Mouse brain cells, using AP2S1 antibody (CAB9263) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
