The AMSH Polyclonal Antibody (CAB20889) is a specialized research tool for studying AMSH, a protein involved in the regulation of protein degradation and trafficking within cells. This antibody is produced in rabbits and has high specificity for human samples, making it ideal for use in Western blot applications to detect and analyze AMSH protein levels.AMSH, also known as associated molecule with the SH3 domain of STAM, is a crucial player in the process of endosomal trafficking and protein turnover. Dysregulation of AMSH activity has been linked to various diseases, including cancer and neurological disorders.
Understanding the role of AMSH in these processes is essential for developing targeted therapies and treatments.Researchers in the fields of cell biology, molecular biology, and biochemistry will find the AMSH Polyclonal Antibody to be a valuable tool in their studies of intracellular protein degradation pathways and cellular signaling mechanisms. With its high sensitivity and specificity, this antibody enables precise detection and analysis of AMSH protein expression in various cell types, contributing to advancements in biomedicine and drug discovery.
Product Name:
AMSH Monoclonal Antibody
SKU:
CAB20889
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Monkey
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 100-200 of human AMSH (O95630).
Sequence:
FPKA EELK AELL KRYT KEYT EYNE EKKK EAEE LARN MAIQ QELE KEKQ RVAQ QKQQ QLEQ EQFH AFEE MIRN QELE KERL KIVQ EFGK VDPG LGGP LVPD L
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:1000
Synonyms:
AMSH; MICCAP
Positive Sample:
293T,HeLa,Jurkat,COS-7
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, Early endosome, Membrane, Nucleus, Peripheral membrane protein.
Calculated MW:
48kDa
Observed MW:
50kDa
Cytokine-mediated signal transduction in the JAK-STAT cascade requires the involvement of adaptor molecules. One such signal-transducing adaptor molecule contains an SH3 domain that is required for induction of MYC and cell growth. The protein encoded by this gene binds to the SH3 domain of the signal-transducing adaptor molecule, and plays a critical role in cytokine-mediated signaling for MYC induction and cell cycle progression. Multiple alternatively spliced transcript variants encoding the same protein isoform have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
10617
Clone Number:
ARC2840
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using AMSH antibody (CAB20889) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
