AMSH Monoclonal Antibody (CAB20889)
- SKU:
- CAB20889
- Product Type:
- Antibody
- Antibody Type:
- Polyclonal Antibody
- Reactivity:
- Plant
- Host Species:
- Rabbit
- Isotype:
- IgG
Frequently bought together:
Description
| Product Name: | AMSH Monoclonal Antibody |
| SKU: | CAB20889 |
| Size: | 20uL, 100uL |
| Isotype: | IgG |
| Host Species: | Rabbit |
| Reactivity: | Human,Monkey |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 100-200 of human AMSH (O95630). |
| Sequence: | FPKA EELK AELL KRYT KEYT EYNE EKKK EAEE LARN MAIQ QELE KEKQ RVAQ QKQQ QLEQ EQFH AFEE MIRN QELE KERL KIVQ EFGK VDPG LGGP LVPD L |
| Tested Applications: | WB ELISA |
| Recommended Dilution: | WB,1:500 - 1:1000 |
| Synonyms: | AMSH; MICCAP |
| Positive Sample: | 293T,HeLa,Jurkat,COS-7 |
| Conjugate: | Unconjugated |
| Cellular Localization: | Cytoplasm, Early endosome, Membrane, Nucleus, Peripheral membrane protein. |
| Calculated MW: | 48kDa |
| Observed MW: | 50kDa |
Cytokine-mediated signal transduction in the JAK-STAT cascade requires the involvement of adaptor molecules. One such signal-transducing adaptor molecule contains an SH3 domain that is required for induction of MYC and cell growth. The protein encoded by this gene binds to the SH3 domain of the signal-transducing adaptor molecule, and plays a critical role in cytokine-mediated signaling for MYC induction and cell cycle progression. Multiple alternatively spliced transcript variants encoding the same protein isoform have been found for this gene.
| Purification Method: | Affinity purification |
| Gene ID: | 10617 |
| Clone Number: | ARC2840 |
| Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3. |
 | Western blot analysis of extracts of various cell lines, using AMSH antibody (CAB20889) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s. |
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