The Anti-Alpha-2-Macroglobulin (A2M) Antibody (CAB9752) is a vital tool for research involving the alpha-2-macroglobulin protein, a major plasma protein that plays a key role in the immune system and inflammation regulation. This polyclonal antibody, produced in rabbits, shows high reactivity with human samples and is validated for use in Western blot applications. By specifically targeting the A2M protein, researchers can accurately detect and analyze its expression in various cell types, making this antibody ideal for studies in immunology, inflammation, and disease research.
Alpha-2-macroglobulin is a versatile protein that acts as a broad-spectrum protease inhibitor, modulating immune responses and inflammatory processes. Its involvement in various physiological and pathological conditions, such as cancer, arthritis, and Alzheimer's disease, underscores its significance as a potential therapeutic target. By delving into the functions of alpha-2-macroglobulin using this antibody, researchers can gain essential insights for developing targeted therapies and interventions in immune-related diseases.
The protein encoded by this gene is a protease inhibitor and cytokine transporter. It uses a bait-and-trap mechanism to inhibit a broad spectrum of proteases, including trypsin, thrombin and collagenase. It can also inhibit inflammatory cytokines, and it thus disrupts inflammatory cascades. Mutations in this gene are a cause of alpha-2-macroglobulin deficiency. This gene is implicated in Alzheimer's disease (AD) due to its ability to mediate the clearance and degradation of A-beta, the major component of beta-amyloid deposits. A related pseudogene, which is also located on the p arm of chromosome 12, has been identified.
Purification Method:
Affinity purification
Gene ID:
2
Clone Number:
ARC1734
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of lysates from Hep G2 cells, using Alpha-2-Macroglobulin (Alpha-2-Macroglobulin (A2M)) Rabbit mAb (CAB9752) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
