The Anti-ALOX5 Antibody (CAB2877) is a valuable tool for researchers studying the ALOX5 enzyme, which plays a crucial role in the metabolism of arachidonic acid and the production of leukotrienes involved in inflammatory responses. This polyclonal antibody, produced in rabbits, is highly specific to human ALOX5 and is validated for use in Western blot applications, allowing for accurate detection and analysis of the enzyme in various cell types.ALOX5 is an important enzyme in the 5-lipoxygenase pathway, which is responsible for the synthesis of leukotrienes that are potent pro-inflammatory mediators in conditions such as asthma, atherosclerosis, and inflammatory bowel disease.
By targeting ALOX5, researchers can gain insights into the mechanisms underlying these diseases and potentially develop new therapeutic strategies to modulate inflammatory responses.The Anti-ALOX5 Antibody (CAB2877) is a valuable tool for investigating the role of ALOX5 in inflammatory processes and identifying potential targets for drug development in the treatment of inflammatory disorders. Its high specificity and sensitivity make it an essential reagent for studies in immunology, pharmacology, and drug discovery.
Product Name:
ALOX5 Rabbit Monoclonal Antibody
SKU:
CAB2877
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 100-200 of human ALOX5 (P09917).
This gene encodes a member of the lipoxygenase gene family and plays a dual role in the synthesis of leukotrienes from arachidonic acid. The encoded protein, which is expressed specifically in bone marrow-derived cells, catalyzes the conversion of arachidonic acid to 5(S)-hydroperoxy-6-trans-8,11,14-cis-eicosatetraenoic acid, and further to the allylic epoxide 5(S)-trans-7,9-trans-11,14-cis-eicosatetrenoic acid (leukotriene A4). Leukotrienes are important mediators of a number of inflammatory and allergic conditions. Mutations in the promoter region of this gene lead to a diminished response to antileukotriene drugs used in the treatment of asthma and may also be associated with atherosclerosis and several cancers. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
240
Clone Number:
ARC1926
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of lysates from Raji cells, using ALOX5 Rabbit mAb (CAB2877) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
