The Anti-AKAP14 Antibody (CAB19818) is a valuable tool for researchers interested in studying AKAP14, a protein known for its role in cellular signaling and regulation. This polyclonal antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blot applications.AKAP14, also known as A-kinase anchor protein 14, is involved in the organization of signaling molecules within cells, particularly in the context of intracellular signaling cascades. By targeting AKAP14 with this antibody, researchers can detect and analyze the protein in various cell types, providing insights into its function and potential implications in different biological processes.
Given the diverse functions of AKAP14 in cellular signaling pathways, this antibody is a valuable tool for studies in molecular biology, cell biology, and biochemistry. Understanding the role of AKAP14 in normal cellular processes and disease states can contribute to the development of novel therapeutic strategies targeting this protein.
Product Name:
AKAP14 Rabbit Monoclonal Antibody
SKU:
CAB19818
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 70-150 of human AKAP14 (Q86UN6).
Sequence:
HGEF TVEK GLKQ IDEY FSKC VSKK CWAH GVEF VERK DLIH SFLY IYYV HWSI STAD LPVA RISA GTYF TMKV SKTK PPDA P
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:1000
Synonyms:
AKAP28; PRKA14; AKAP14
Positive Sample:
A-549,DU 145,Mouse testis, Rat testis
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm.
Calculated MW:
23kDa
Observed MW:
23kDa
The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The protein anchors PKA in ciliary axonemes and, in this way, may play a role in regulating ciliary beat frequency. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Purification Method:
Affinity purification
Gene ID:
158798
Clone Number:
ARC2344
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using AKAP14 Rabbit mAb (CAB19818) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3s.
