The Anti-ACTN3 Antibody (CAB19631) offered by AssayGenie is a high-quality polyclonal antibody designed for research involving ACTN3, a protein primarily found in skeletal muscle that plays a critical role in muscle function and performance. This antibody, generated in rabbits, exhibits high reactivity with human samples and is validated for use in various applications, including Western blotting. By binding specifically to the ACTN3 protein, this antibody enables accurate detection and analysis in a variety of cell types, making it an excellent tool for studying muscle biology, exercise physiology, and sports genetics.
ACTN3 is known as the "sprinter gene" due to its association with sprinting and power performance in athletes. Understanding the role of ACTN3 in muscle function and performance can provide valuable insights into muscle-related disorders, sports performance, and exercise training strategies. This antibody is a valuable resource for researchers looking to explore the functions and mechanisms of ACTN3 in both health and disease contexts.
Product Name:
ACTN3 Rabbit Monoclonal Antibody
SKU:
CAB19631
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 700-800 of human ACTN3 (Q08043).
Sequence:
DRLE GDHQ LLQE SLVF DNKH TVYS MEHI RVGW EQLL TSIA RTIN EVEN QVLT RDAK GLSQ EQLN EFRA SFNH FDRK QNGM MEPD DFRA CLIS MGYD LGEV E
Tested Applications:
WBIF/ICCELISA
Recommended Dilution:
WB,1:500 - 1:1000 IF/ICC,1:50 - 1:200
Synonyms:
ACTN3D; ACTN3
Positive Sample:
Mouse skeletal muscle,Rat skeletal muscle
Conjugate:
Unconjugated
Cellular Localization:
Cortical actin cytoskeleton, Cytosol, Extracellular exosome, Plasma membrane, Z disc
Calculated MW:
103kDa
Observed MW:
103kDa
This gene encodes a member of the alpha-actin binding protein gene family. The encoded protein is primarily expressed in skeletal muscle and functions as a structural component of sarcomeric Z line. This protein is involved in crosslinking actin containing thin filaments. An allelic polymorphism in this gene results in both coding and non-coding variants; the reference genome represents the coding allele. The non-functional allele of this gene is associated with elite athlete status.
Purification Method:
Affinity purification
Gene ID:
89
Clone Number:
ARC2202
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using ACTN3 Rabbit mAb (CAB19631) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
