The Anti-Acetyl Histone H3 (K36) Antibody (CAB20185) is a powerful tool for researchers studying epigenetics and chromatin modification. This specific antibody, produced in rabbits, is highly specific for detecting acetylation at lysine 36 on histone H3 in a variety of human samples. Validated for use in Western blot applications, this antibody enables researchers to investigate the role of acetyl histone H3 (K36) in gene expression regulation and chromatin remodeling.Histone modifications, such as acetylation of lysine residues, play a crucial role in controlling gene expression by altering chromatin structure. Acetyl histone H3 (K36) is associated with active transcription and is involved in various biological processes, including DNA repair and cell differentiation.
By targeting this specific histone modification, researchers can gain valuable insights into the epigenetic mechanisms underlying diseases such as cancer, neurodegenerative disorders, and developmental abnormalities.The Anti-Acetyl Histone H3 (K36) Antibody (CAB20185) is a valuable tool for studying the dynamic interplay between histone modifications and gene regulation, providing researchers with the ability to explore the epigenetic landscape in health and disease. Its high sensitivity and specificity make it an essential reagent for investigating epigenetic changes and their impact on cellular function.
Product Name:
Acetyl-Histone H3-K36 Rabbit Polyclonal Antibody
SKU:
CAB20185
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat,Other (Wide Range Predicted)
Immunogen:
A synthetic acetylated peptide around K36 of human Histone H3 (NP_003520.1).
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.
Purification Method:
Affinity purification
Gene ID:
8290/8350
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from C6 cells, using Acetyl-Histone H3-K36 Rabbit pAb (CAB20185) at 1:1000 dilution.C6 cells were treated by TSA (1 uM) at 37℃ for 18 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 180s.
