The Anti-Acetyl Histone H3 (K27) Antibody (CAB20184) is a valuable tool for researchers studying epigenetics and chromatin regulation. This polyclonal antibody, raised in rabbits, specifically targets the acetylated lysine 27 residue on Histone H3, a crucial modification involved in gene expression and chromatin structure.Histone acetylation at K27 is associated with transcriptional activation and is often found at active gene promoters. This antibody is highly reactive with human samples and has been validated for use in applications such as Western blotting, chromatin immunoprecipitation (ChIP), and immunofluorescence microscopy, providing researchers with versatile options for their experiments.
By detecting and analyzing the acetylation status of Histone H3 at K27, researchers can gain valuable insights into gene regulation, chromatin dynamics, and disease mechanisms. This antibody is ideal for studies in fields such as cancer biology, developmental biology, and epigenetics, where epigenetic modifications play a critical role in cellular processes.Overall, the Anti-Acetyl Histone H3 (K27) Antibody (CAB20184) is a reliable and specific tool for investigating the role of histone acetylation in gene expression and chromatin remodeling processes, paving the way for a deeper understanding of epigenetic regulation in health and disease.
Product Name:
Acetyl-Histone H3-K27 Rabbit Polyclonal Antibody
SKU:
CAB20184
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat,Other (Wide Range Predicted)
Immunogen:
A synthetic acetylated peptide around K27 of human Histone H3 (NP_003520.1).
Sequence:
ARKS A
Tested Applications:
WBChIPELISADB
Recommended Dilution:
DB,1:500 - 1:1000 WB,1:500 - 1:1000 ChIP,5μg antibody for 5μg-10μg of Chromatin
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.
Purification Method:
Affinity purification
Gene ID:
8290/8350
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates using Acetyl-Histone H3-K27 Rabbit pAb (CAB20184) at 1:1000 dilution.NIH/3T3 cells were treated by TSA (1 uM) at 37℃ for 18 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
