The Anti-Acetyl BRD4 (K332) Antibody (CAB20208) is a valuable tool for researchers studying epigenetic regulation and chromatin remodeling. This antibody, generated in rabbits, specifically targets the acetylated lysine 332 residue on the BRD4 protein, allowing for precise detection and analysis in various experimental settings.BRD4 is a key player in the regulation of gene expression through its interaction with acetylated histones, and specifically acetylated lysine 332 has been implicated in controlling transcriptional activation. The Anti-Acetyl BRD4 (K332) Antibody enables researchers to investigate the role of this post-translational modification in gene regulation, making it an essential component in epigenetics and cancer research.
By utilizing this antibody in techniques such as Western blotting, immunoprecipitation, and immunofluorescence, researchers can gain insights into the mechanisms underlying BRD4 function and its implications in various disease states. The information obtained from studying BRD4 acetylation at lysine 332 can lead to the development of novel therapeutic approaches targeting epigenetic dysregulation in cancer and other diseases.
Product Name:
Acetyl-BRD4-K332 Rabbit Polyclonal Antibody
SKU:
CAB20208
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
A synthetic acetylated peptide around K332 of human BRD4 (NP_490597.1).
Sequence:
PPKK D
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:1000
Synonyms:
CAP; MCAP; HUNK1; HUNKI; FSHRG4; Acetyl-BRD4-K332
Positive Sample:
Mouse brain
Cellular Localization:
Chromosome, Nucleus.
Calculated MW:
152kDa
Observed MW:
200kDa
The protein encoded by this gene is homologous to the murine protein MCAP, which associates with chromosomes during mitosis, and to the human RING3 protein, a serine/threonine kinase. Each of these proteins contains two bromodomains, a conserved sequence motif which may be involved in chromatin targeting. This gene has been implicated as the chromosome 19 target of translocation t(15;19)(q13;p13.1), which defines an upper respiratory tract carcinoma in young people. Two alternatively spliced transcript variants have been described.
Purification Method:
Affinity purification
Gene ID:
23476
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from Mouse brain, using Acetyl-BRD4-K332 Rabbit pAb (CAB20208) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
