The Anti-5-Methylcytosine (5mC) Antibody (CAB20599) is a highly specific and sensitive tool for studying epigenetic modifications related to DNA methylation. This monoclonal antibody, produced in mouse, is optimized for detecting 5mC in various genomic DNA samples, making it a valuable asset for researchers in the fields of epigenetics and molecular biology.DNA methylation at the 5th position of cytosine residues (5mC) plays a crucial role in gene expression regulation, genomic stability, and cellular differentiation. Aberrant DNA methylation patterns have been linked to various diseases, including cancer, neurological disorders, and developmental abnormalities. The Anti-5-Methylcytosine (5mC) Antibody enables researchers to explore these epigenetic modifications and their impact on cellular processes, providing insights into disease mechanisms and potential therapeutic targets.
Whether investigating the role of DNA methylation in cancer progression, studying epigenetic changes during cellular reprogramming, or exploring the effects of environmental factors on gene regulation, the Anti-5-Methylcytosine (5mC) Antibody offers a reliable and versatile tool for epigenetic research. Its high specificity and sensitivity make it an essential component of any laboratory studying the complex interplay between DNA methylation and gene expression.
Product Name:
5-Methylcytosine (5mC) Rabbit Monoclonal Antibody
SKU:
CAB20599
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Species independent
Immunogen:
5mC
Sequence:
5mC conj ugat ed t o KL H
Tested Applications:
ELISADB
Recommended Dilution:
DB,1:500 - 1:1000
Synonyms:
5mC; 5-Methylcytosine (5mC)
Positive Sample:
5-Methylcytosine (5mC)
In the mammalian genome, DNA methylation is an epigenetic mechanism involving the transfer of a methyl group onto the C5 position of the cytosine to form 5-methylcytosine. DNA methylation regulates gene expression by recruiting proteins involved in gene repression or by inhibiting the binding of transcription factor(s) to DNA. During development, the pattern of DNA methylation in the genome changes as a result of a dynamic process involving both de novo DNA methylation and demethylation. As a consequence, differentiated cells develop a stable and unique DNA methylation pattern that regulates tissue-specific gene transcription.? Intriguingly, postmitotic neurons still express DNA methyltransferases and components involved in DNA demethylation. Moreover, neuronal activity can modulate their pattern of DNA methylation in response to physiological and environmental stimuli. The precise regulation of DNA methylation is essential for normal cognitive function.
Purification Method:
Affinity purification
Clone Number:
ARC50801
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Dot-blot analysis of 5-Methylcytosine (5mC) and unmodified cytosine using 5-Methylcytosine (5mC) Rabbit mAb antibody (CAB20599) at 1:1000 dilution.5-Methylcytosine (5mC) : Biotin-5'CGATAACCACTAGT(5mC)3'unmodified cytosine : Biotin-5'CGATAACCACTAGTC3'
