The Anti-2'-O-Methyluridine (Um) Antibody (CAB20305) from Assay Genie is a high-quality tool for researchers studying RNA modifications. This polyclonal antibody, raised in rabbits, is highly specific for 2'-O-methyluridine (Um) and has been validated for use in various applications, including Western blotting and immunohistochemistry.2'-O-methyluridine (Um) is a modified nucleoside found in various RNA species, and its presence can impact RNA structure and function. Studying Um modifications can provide insights into RNA biology, including RNA stability, translation efficiency, and interactions with RNA-binding proteins.
With its high specificity and validated performance, the Anti-2'-O-Methyluridine (Um) Antibody is an essential tool for researchers investigating RNA modifications and their functional consequences. This antibody's ability to detect and analyze Um modifications in different experimental settings makes it a valuable resource for studies in molecular biology, RNA biochemistry, and RNA modification dynamics.
Product Name:
2'-O-methyluridine(Um) Rabbit Polyclonal Antibody
SKU:
CAB20305
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Species independent
Immunogen:
Um
Sequence:
Um c onju gate d to KLH
Tested Applications:
ELISADB
Recommended Dilution:
DB,1:500 - 1:2000
Synonyms:
Um; 2'-O-methyluridine; 2'-O-methyluridine(Um)
Conjugate:
Unconjugated
Observed MW:
Refer to figures
RNA methylation plays a significant regulatory role in various of physiological activities and it has gradually become a hotspot of epigenetics in the past decade. 2'-O-methyladenosine (Am), 2'-O-methylguanosine (Gm),2'-O-methylcytidine (Cm),2'-O-methyluridine (Um),N 6-methyladenosine (m6A), N 1-methylguanosine (m1G),5-methylcytidine (m5C),and 5-methyluridine (m5U) are representative 2'-O-methylation and base-methylation modified epigenetic marks of RNA. 2'-O-methyltransferase is a modified nucleoside that is produced in tRNAs by the action of tRNA guanosine-2'-O-methyltransferase, using S-adenosyl-L-methionine as a substrate. Through its interaction with other modified nucleosides, 2'-O-methylguanosine is thought to stabilize the structure of the tRNA.
Purification Method:
Affinity purification
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
The 2'-O-methyluridine(Um) Rabbit pAb(CAB20305) are tested in Dot Blot against 2'-O-methyluridine(Um) and unmodified uridine.Um-3：5'Biotin-UGACAACUACAGAC(Um)3' Um-4：5'Biotin-UGACAACUACAGACU3‘
