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14-3-3 beta/Theta Rabbit Monoclonal Antibody (CAB9156)

SKU:
CAB9156
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Synonyms:
GW128
HEL-S-1
HS1
KCIP-1
YWHAA
Research Area:
Immunology
$167 - $503
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:14-3-3 beta/Theta Rabbit Monoclonal Antibody
SKU:CAB9156
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-100 of human 14-3-3 beta/zeta (P31946).
Sequence:MTMD KSEL VQKA KLAE QAER YDDM AAAM KAVT EQGH ELSN EERN LLSV AYKN VVGA RRSS WRVI SSIE QKTE RNEK KQQM GKEY REKI EAEL QDIC NDVL
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:1000
Synonyms:GW128; HEL-S-1; HS1; KCIP-1; YWHAA; 14-3-3-beta/zeta
Positive Sample:HT-29,A549,NIH/3T3,Jurkat,Mouse brain,Mouse kidney,Rat lung,Rat brain,Rat spleen
Conjugate:Unconjugated
Cellular Localization:cytoplasm, cytosol, extracellular exosome, focal adhesion, melanosome, perinuclear region of cytoplasm
Calculated MW:28kDa
Observed MW:28kDa

This gene encodes a protein belonging to the 14-3-3 family of proteins, members of which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals. The encoded protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Two transcript variants, which encode the same protein, have been identified for this gene. [provided by RefSeq, Jul 2008]

Purification Method:Affinity purification
Gene ID:7529/7534
Clone Number:ARC1455
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Product ImageWestern blot analysis of extracts of various cell lines, using 14-3-3 beta/zeta antibody (CAB9156) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.

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