The Anoctamin-1 Polyclonal Antibody (CAB10498) is a research tool designed for studying the Anoctamin-1 protein, which plays a role in various cellular functions. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding to the Anoctamin-1 protein, this antibody enables detection and analysis in a range of cell types, making it an ideal tool for research in the fields of cell biology, neuroscience, and physiology.Anoctamin-1, also known as TMEM16A, is a calcium-activated chloride channel that regulates processes such as neuronal excitability, smooth muscle contraction, and epithelial secretion.
Understanding the function and regulation of Anoctamin-1 is important for elucidating its roles in various physiological and pathological conditions, including neurological disorders, smooth muscle diseases, and cystic fibrosis.This antibody offers researchers a valuable tool for investigating the complex roles of Anoctamin-1 in cellular physiology and disease mechanisms. Its specificity and reliability make it a useful addition to studies seeking to unravel the intricate functions of this important protein.
Product Name:
ANO1 Rabbit Polyclonal Antibody
SKU:
CAB10498
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 400-500 of human ANO1 (NP_060513.5).
Enables calcium activated cation channel activity; intracellular calcium activated chloride channel activity; and iodide transmembrane transporter activity. Involved in cation transport; inorganic anion transport; and positive regulation of insulin secretion involved in cellular response to glucose stimulus. Located in apical plasma membrane and nucleoplasm.
Purification Method:
Affinity purification
Gene ID:
55107
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates using ANO1 Rabbit pAb (CAB10498) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
