The ANO4 Polyclonal Antibody (PAC035890) is a valuable tool for researchers studying ANO4, a member of the Anoctamin family of proteins known for their calcium-activated chloride channel activity. This antibody, produced in rabbits, demonstrates high reactivity with human samples and has been validated for use in Western blot applications. By targeting the ANO4 protein, this antibody enables the detection and analysis of ANO4 expression in various cell types, making it ideal for studies in ion channel physiology and related fields.
ANO4, also known as TMEM16D, plays a crucial role in chloride ion transport and regulation of cellular excitability. Its involvement in ion channel function makes it a compelling target for research into conditions such as cystic fibrosis, epilepsy, and cardiac arrhythmias. Investigating the function of ANO4 can provide valuable insights into the mechanisms of ion channel regulation and may lead to the development of novel therapeutic strategies for treating ion channel disorders.
Immunohistochemistry of paraffin-embedded human lung cancer using PACO35890 at dilution of 1:100.
Immunofluorescent analysis of Hela cells using PACO35890 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunohistochemistry of paraffin-embedded human adrenal gland tissue using PACO35890 at dilution of 1:100.
Background:
Has calcium-dependent phospholipid scramblase activity; scrambles phosphatidylserine, phosphatidylcholine and galactosylceramide. Does not exhibit calcium-activated chloride channel (CaCC) activity.
Synonyms:
Anoctamin-4 (Transmembrane protein 16D), ANO4, TMEM16D
UniProt Protein Function:
ANO4: May act as a calcium-activated chloride channel. Belongs to the anoctamin family. 3 isoforms of the human protein are produced by alternative splicing.Protein type: Transporter, ion channel; Transporter; Membrane protein, multi-pass; Membrane protein, integralChromosomal Location of Human Ortholog: 12q23.1Cellular Component: intracellular; plasma membraneMolecular Function: intracellular calcium activated chloride channel activityBiological Process: chloride transport
