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Annexin V-Biotin Apoptosis Detection Kit (CV0003)

SKU:
CV0003
Product Type:
Assay
Detection Method:
Fluorometric
Sample Type:
Adherent Cells, Suspension Cells
Research Area:
Apoptosis
Annexin
$335
Frequently bought together:

Description

ELISA Kit Technical ManualMSDS

Annexin V-Biotin Apoptosis Detection Kit

Annexin V Apoptosis Detection Kits are a convenient, easy-to-use and safe method for Apoptosis Detection. Annexins are a family of calcium-dependent phospholipid-binding proteins, which bind to phosphatidylserine (PS).

Externalization of phosphatidylserine residues on the outer plasma membrane of apoptotic cells allows detection via Annexin V. Once the apoptotic cells are bound with labelled Annexin V, it can be visualized with fluorescent microscopy or cytometry.

Since loss of membrane integrity is a pathognomonic feature of necrotic cell death, necrotic cells will stain with specific membrane-impermeant nucleic acid dyes such as propidium iodide, the membrane integrity of apoptotic cells can be demonstrated by the exclusion of these dyes.

Features & Benefits

Overview Description

Size:

100 Assays

Easy and one step protocol:

Get results in 9 minutes.

Detection Method:

Flow cytometry (Ex = 488 nm; Em = 530 nm) and fluorescence microscopy.

Reactivity:

Human, Mouse, Rat & other species

Proven performance:

Works with both adherent and suspension cells.

Safe:

Non-enzymatic assay that avoids fixation.

Reagents Included:

Buffer/Reagent Volume

Labeled Annexin V

500 µL

Binding Buffer (10x)

50 mL

Propidium Iodide

1 mL

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