Annexin A4/Annexin IV Rabbit Polyclonal Antibody (CAB6280)
The Annexin A4 Polyclonal Antibody (CAB6280) is a valuable tool for researchers studying Annexin A4, a protein involved in calcium-dependent membrane binding and exocytosis. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in Western blotting applications. By binding specifically to Annexin A4, this antibody enables precise detection and analysis of the protein in various cell types, making it an essential component for studies in cell biology and neuroscience.Annexin A4 is known for its role in regulating membrane fusion events and vesicle trafficking, making it a crucial player in cellular processes such as neurotransmitter release and cell signaling.
Its involvement in calcium-dependent pathways also suggests its potential importance in diseases related to cellular dysfunction and signaling dysregulation. Studying Annexin A4 with this antibody can help researchers uncover novel mechanisms underlying these disorders and potentially identify new therapeutic targets for intervention.
Product Name:
Annexin A4/Annexin IV Rabbit Polyclonal Antibody
SKU:
CAB6280
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 112-321 of human Annexin A4/Annexin IV (NP_001144.1).
cell surface, cytoplasm, cytoplasmic vesicle, extracellular exosome, nuclear membrane, nucleus, perinuclear region of cytoplasm, plasma membrane
Calculated MW:
36kDa
Observed MW:
35kDa
Annexin IV (ANX4) belongs to the annexin family of calcium-dependent phospholipid binding proteins. Although their functions are still not clearly defined, several members of the annexin family have been implicated in membrane-related events along exocytotic and endocytotic pathways. ANX4 has 45 to 59% identity with other members of its family and shares a similar size and exon-intron organization. Isolated from human placenta, ANX4 encodes a protein that has possible interactions with ATP, and has in vitro anticoagulant activity and also inhibits phospholipase A2 activity. ANX4 is almost exclusively expressed in epithelial cells. Several transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
307
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using Annexin A4/Annexin IV antibody (CAB6280) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 60s.
