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Angiotensin Converting Enzyme (ACE) Activity Assay Kit (MAES0334)

SKU:
MAES0334
Product Type:
Assay Kit
Size:
100 Assays
Detection Method:
Colorimetric Method
Sensitivity:
27.5 U/L
Range:
27.5-682 U/L
$563

Description

Product Name: Angiotensin Converting Enzyme (ACE) Activity Assay Kit
SKU: MAES0334
Size: 100 Assays
Detection Method: Colorimetric method
Synonyms: ACE1
Sensitivity: 27.5 U/L
Range: 27.5-682 U/L
Detection Instrument: Spectrophotometer (340 nm)
Sample Type: Serum, Plasma, Animal Tissue
Other Reagents Required: PBS (0.01 mol/L, pH 7.4)
Precision: inter-assay CV: 4.4% | intra-assay CV: 0.03
Assay Time: 50 minutes
Storage: This product can be stored at 2-8°C for 12 months with shading light.
Detection Principle: N-[3-(2-furyl)acryloyl]-L-phenylalanylglycylglycine (FAPGG) have the maximum absorption peak at 340 nm, angiotensin converting enzyme catalyze N-(3[2-Furyl ]Acryloyl)-Phe-Gly-Gly to produce FAP and GG, and the absorbance at 340 nm will be decreased. The activity of ACE1 can be calculated indirectly by measuring the decrease in absorbance at 340 nm.
Sample Dilution Reference:
Sample Type Dilution Factor
Human Serum 1
Mouse Serum 1
Rat Plasma 1
10% Rat liver tissue homogenate 1
10% Rat kidney tissue homogenate 1
10% Rat lung tissue homogenate 3-5

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