The Angiogenin Polyclonal Antibody (CAB1546) is a valuable tool for researchers studying angiogenin, a protein involved in the regulation of angiogenesis and cell growth. This antibody, generated in rabbits, is highly specific for human samples and has been validated for use in Western blot applications.Angiogenin is a key player in neovascularization processes, making it a potential target for research in cancer, cardiovascular disease, and other conditions involving abnormal blood vessel growth.
The Angiogenin Polyclonal Antibody binds to the angiogenin protein, allowing for its detection and analysis in various cell types.By elucidating the role of angiogenin in disease processes, researchers can uncover potential therapeutic targets and develop treatments aimed at modulating angiogenesis. The Angiogenin Polyclonal Antibody provides a reliable tool for investigating the mechanisms underlying angiogenin function, facilitating advancements in the field of angiogenesis research.
Product Name:
ANG Rabbit Polyclonal Antibody
SKU:
CAB1546
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 25-147 of human ANG (NP_001136.1).
The protein encoded by this gene is a member of the RNase A superfamily though it has relatively weak ribonucleolytic activity. This protein is a potent mediator of new blood vessel formation and thus, in addition to the name RNase5, is commonly called angiogenin. This protein induces angiogenesis after binding to actin on the surface of endothelial cells. This protein also accumulates at the nucleolus where it stimulates ribosomal transcription. Under stress conditions this protein translocates to the cytosol where it hydrolyzes cellular tRNAs and influences protein synthesis. A signal peptide is cleaved from the precursor protein to produce a mature protein which contains a nuclear localization signal, a cell binding motif, and a catalytic domain. This protein has been shown to be both neurotrophic and neuroprotective and the mature protein has antimicrobial activity against some bacteria and fungi, including S. pneumoniae and C. albicans. Due to its effect on rRNA production and angiogenesis this gene plays important roles in cell growth and tumor progression. Mutations in this gene are associated with progression of amyotrophic lateral sclerosis (ALS). This gene and the neighboring RNase4 gene share promoters and 5' exons though each gene then splices to a distinct 3' exon containing the complete coding region of each gene. Alternative splicing results in multiple transcript variants encoding the same protein.
Purification Method:
Affinity purification
Gene ID:
283
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates, using ANG Rabbit pAb (CAB1546) at 1:700 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 60s.
