The ANG Polyclonal Antibody (CAB13465) is a valuable tool for researchers studying angiogenin (ANG), a protein involved in angiogenesis, inflammation, and tumorigenesis. This antibody, raised in rabbits, specifically targets ANG and has been validated for use in various applications, including Western blot and immunohistochemistry.Angiogenin is a potent inducer of new blood vessel formation, making it a key player in processes such as wound healing, tissue repair, and tumor growth.
By targeting ANG with this antibody, researchers can gain insights into its role in angiogenesis and its potential as a therapeutic target for conditions such as cancer and cardiovascular diseases.With its high reactivity and specificity for ANG, the ANG Polyclonal Antibody (CAB13465) is an essential tool for researchers looking to unravel the complexities of angiogenin biology and its implications for human health.
Product Name:
ANG Rabbit Polyclonal Antibody
SKU:
CAB13465
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 25-147 of human ANG (NP_001136.1).
The protein encoded by this gene is a member of the RNase A superfamily though it has relatively weak ribonucleolytic activity. This protein is a potent mediator of new blood vessel formation and thus, in addition to the name RNase5, is commonly called angiogenin. This protein induces angiogenesis after binding to actin on the surface of endothelial cells. This protein also accumulates at the nucleolus where it stimulates ribosomal transcription. Under stress conditions this protein translocates to the cytosol where it hydrolyzes cellular tRNAs and influences protein synthesis. A signal peptide is cleaved from the precursor protein to produce a mature protein which contains a nuclear localization signal, a cell binding motif, and a catalytic domain. This protein has been shown to be both neurotrophic and neuroprotective and the mature protein has antimicrobial activity against some bacteria and fungi, including S. pneumoniae and C. albicans. Due to its effect on rRNA production and angiogenesis this gene plays important roles in cell growth and tumor progression. Mutations in this gene are associated with progression of amyotrophic lateral sclerosis (ALS). This gene and the neighboring RNase4 gene share promoters and 5' exons though each gene then splices to a distinct 3' exon containing the complete coding region of each gene. Alternative splicing results in multiple transcript variants encoding the same protein.
Purification Method:
Affinity purification
Gene ID:
283
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using ANG Rabbit pAb (CAB13465) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3min.
