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Androgen Receptor (Phospho-Ser650) Transcription Factor Activity Assay

SKU:
TFAB00012
Product Type:
ELISA Kit
ELISA Type:
Transcription Factor Assay
Analytes:
Androgen Receptor (Phospho-Ser650)
Reactivity:
Human
Mouse
Rat
€599
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Description

system_update_altDatasheet

Androgen Receptor (Phospho-Ser650)Transcription Factor Activity Assay

The Androgen Receptor Phospho-Ser650 Transcription Factor Activity Assay is a cutting-edge kit designed for the detection and quantification of androgen receptor activity in cells. This assay is highly sensitive and specific, allowing for accurate measurement of transcription factor activity related to the phosphorylation of serine 650 on the androgen receptor.The androgen receptor is a critical protein in the regulation of gene expression related to male sexual development and other physiological processes. Phosphorylation of serine 650 on the androgen receptor is known to influence its transcriptional activity, making this assay a valuable tool for researchers studying hormone signaling pathways, cancer biology, and other related fields.

By providing researchers with a reliable and reproducible method for assessing androgen receptor activity, the Androgen Receptor Phospho-Ser650 Transcription Factor Activity Assay is essential for advancing our understanding of androgen receptor function and developing potential therapies for diseases related to hormone signaling.

Product Name:Androgen Receptor (Phospho-Ser650) Transcription Factor Activity Assay
Product Code:TFAB00012
Target:Androgen Receptor (Phospho-Ser650)
Synonyms:Dihydrotestosterone receptor, Nuclear receptor subfamily 3 group C member 4
Reactivity:Human, Mouse, Rat
Sample Types:Nuclear or cell lysates

The Assay Genie Androgen Receptor (Phospho-Ser650) transcription factor activity assay allows for the detection and qualitative analysis of Androgen Receptor (Phospho-Ser650) active transcription factors in eukaryotic nuclear or cell lysates.

Assay Genie ELISA kits are designed to significantly reduce experiment time and ensure sensitivity and flexibility for high-throughput screening.

Assay Time:4.5 hours
Detection Method:Colorimetric 450 nm
Size:12 x 8-Well Microstrips
Storage:4°C for 6 months
UniProt Protein Function:AR: a nuclear hormone receptor and transcription factor. Regulates gene expression and affects cellular proliferation and differentiation in target tissues. Two splice-variant isoforms have been described.
UniProt Protein Details:

Protein type:Transcription factor; Nuclear receptor; DNA-binding

Chromosomal Location of Human Ortholog: Xq12

Cellular Component: nucleoplasm; protein complex; cytoplasm; nuclear chromatin; nucleus

Molecular Function:protein dimerization activity; protein binding; ligand-dependent nuclear receptor activity; androgen receptor activity; enzyme binding; DNA binding; androgen binding; zinc ion binding; beta-catenin binding; chromatin binding; transcription factor binding; transcription factor activity; receptor binding

Biological Process: prostate gland development; transcription initiation from RNA polymerase II promoter; intracellular receptor-mediated signaling pathway; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; signal transduction; protein oligomerization; activation of NF-kappaB transcription factor; negative regulation of integrin biosynthetic process; cell proliferation; cell-cell signaling; transport; androgen receptor signaling pathway; positive regulation of cell proliferation; positive regulation of transcription from RNA polymerase III promoter; gene expression; steroid hormone mediated signaling; positive regulation of transcription from RNA polymerase II promoter; positive regulation of integrin biosynthetic process; cell growth; sex differentiation; positive regulation of phosphorylation

Disease: Androgen Insensitivity Syndrome; Prostate Cancer; Androgen Insensitivity, Partial; Hypospadias 1, X-linked; Spinal And Bulbar Muscular Atrophy, X-linked 1

NCBI Summary:The androgen receptor gene is more than 90 kb long and codes for a protein that has 3 major functional domains: the N-terminal domain, DNA-binding domain, and androgen-binding domain. The protein functions as a steroid-hormone activated transcription factor. Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. This gene contains 2 polymorphic trinucleotide repeat segments that encode polyglutamine and polyglycine tracts in the N-terminal transactivation domain of its protein. Expansion of the polyglutamine tract causes spinal bulbar muscular atrophy (Kennedy disease). Mutations in this gene are also associated with complete androgen insensitivity (CAIS). Two alternatively spliced variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]
UniProt Code:P10275
NCBI GenInfo Identifier:113830
NCBI Gene ID:367
NCBI Accession:P10275.2
UniProt Secondary Accession:P10275,Q9UD95, A2RUN2, B1AKD7,
UniProt Related Accession:P10275
Molecular Weight:44,643 Da
NCBI Full Name:Androgen receptor
NCBI Synonym Full Names:androgen receptor
NCBI Official Symbol:AR  
NCBI Official Synonym Symbols:KD; AIS; TFM; DHTR; SBMA; HYSP1; NR3C4; SMAX1; HUMARA  
NCBI Protein Information:androgen receptor; androgen nuclear receptor variant 2; dihydrotestosterone receptor; nuclear receptor subfamily 3 group C member 4
UniProt Protein Name:Androgen receptor
UniProt Synonym Protein Names:Dihydrotestosterone receptor; Nuclear receptor subfamily 3 group C member 4
Protein Family:Allatostatin
UniProt Gene Name:AR  
UniProt Entry Name:ANDR_HUMAN
AntibodiesELISA Kits
Anti-Androgen Receptor Antibody (CAB0330)Human AR (Androgen Receptor) ELISA Kit (HUES01695)
Anti-Androgen Receptor Antibody (CAB16200)Androgen Receptor (Phospho-Tyr363) Transcription Factor Activity Assay
Anti-Androgen Receptor Antibody (CAB19611)Androgen Receptor Transcription Factor Activity Assay
Anti-Androgen Receptor Antibody (CAB2053)
Anti-Phospho-Androgen receptor-S213 Antibody (CABP0306)

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