ANAPC5 Rabbit Polyclonal Antibody (CAB7109)
- SKU:
- CAB7109
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Cycle
Description
Product Name: | ANAPC5 Rabbit Polyclonal Antibody |
SKU: | CAB7109 |
Size: | 20uL, 100uL |
Isotype: | IgG |
Host Species: | Rabbit |
Reactivity: | Human,Mouse,Rat |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human ANAPC5 (NP_057321.2). |
Sequence: | MASV HESL YFNP MMTN GVVH ANVF GIKD WVTP YKIA VLVL LNEM SRTG EGAV SLME RRRL NQLL LPLL QGPD ITLS KLYK LIEE SCPQ LANS VQIR IKLM AEGE LKDM EQFF DDLS DSFS GTEP EVHK TSVV GLFL RHMI LAYS KLSF SQVF KLYT ALQQ YFQN GEKK TVED ADME LTSR DEGE RKME KEEL DVSV REEE VSCS GPLS QKQA EFFL SQQA SLLK NDET KALT PASL QKEL NNLL KFNP DF |
Tested Applications: | WB IHC-P IP ELISA |
Recommended Dilution: | WB,1:500 - 1:1000 IHC-P,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells |
Synonyms: | APC5; C5 |
Positive Sample: | 293T,SW620,MCF-7,HL-60,22Rv1,A-549,Mouse spleen |
Conjugate: | Unconjugated |
Cellular Localization: | cytosol, nucleoplasm, nucleus, spindle |
Calculated MW: | 85kDa |
Observed MW: | 85kDa |
This gene encodes a tetratricopeptide repeat-containing component of the anaphase promoting complex/cyclosome (APC/C), a large E3 ubiquitin ligase that controls cell cycle progression by targeting a number of cell cycle regulators such as B-type cyclins for 26S proteasome-mediated degradation through ubiquitination. The encoded protein is required for the proper ubiquitination function of APC/C and for the interaction of APC/C with transcription coactivators. It also interacts with polyA binding protein and represses internal ribosome entry site-mediated translation. Multiple transcript variants encoding different isoforms have been found for this gene. These differences cause translation initiation at a downstream AUG and result in a shorter protein (isoform b), compared to isoform a.
Purification Method: | Affinity purification |
Gene ID: | 51433 |
Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3. |