The AMPKa1/AMPKa2 Polyclonal Antibody (CAB17289) is a valuable tool for researchers studying the AMP-activated protein kinase (AMPK) pathway, a key regulator of cellular energy balance and metabolism. This antibody, produced in rabbits, is highly specific for detecting AMPKa1 and AMPKa2 proteins in human samples, making it suitable for use in Western blot applications.AMPK is a central player in maintaining cellular energy homeostasis and is involved in various physiological processes, including glucose uptake, lipid metabolism, and autophagy. Dysregulation of the AMPK pathway has been linked to metabolic disorders, cancer, and cardiovascular diseases, highlighting the importance of studying this pathway for therapeutic interventions.
By utilizing the AMPKa1/AMPKa2 Polyclonal Antibody, researchers can accurately detect and quantify AMPKa1 and AMPKa2 protein levels in different cell types and tissues, providing insights into the role of AMPK in health and disease. This antibody is a valuable tool for investigating the molecular mechanisms underlying metabolic disorders and developing novel therapies targeting the AMPK pathway.
Product Name:
AMPKa1/AMPKa2 Polyclonal Antibody
SKU:
CAB17289
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 5-280 of human AMPKa1/AMPKa2 (NP_006242.5).
The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
Purification Method:
Affinity purification
Gene ID:
5562/5563
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using AMPKa1/AMPKa2 antibody (CAB17289) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
