The AMPKα1/AMPKα2 Polyclonal Antibody (CAB12718) is an essential tool for researchers studying the AMP-activated protein kinase (AMPK) signaling pathway. This antibody, generated in rabbits, is highly specific for both AMPKα1 and AMPKα2 isoforms, allowing for robust detection and analysis in various experimental settings.AMPK is a key regulator of cellular energy homeostasis and metabolism, making it a critical player in numerous physiological processes, including glucose and lipid metabolism, autophagy, and cellular growth. Dysregulation of AMPK activity has been implicated in various diseases, such as diabetes, obesity, and cancer, highlighting the importance of studying this pathway for therapeutic interventions.The AMPKα1/AMPKα2 Polyclonal Antibody is validated for use in Western blot applications, enabling researchers to accurately assess the expression levels and activation states of AMPK in cell lysates or tissue samples.
Its high specificity and sensitivity make it an ideal tool for investigating the roles of AMPK in different cellular and disease contexts.By utilizing this antibody, researchers can gain valuable insights into the function of AMPKα1 and AMPKα2 isoforms, furthering our understanding of their roles in health and disease. Ultimately, the AMPKα1/AMPKα2 Polyclonal Antibody offers a powerful tool for elucidating the mechanisms underlying AMPK signaling and uncovering novel therapeutic targets for various metabolic and metabolic-related disorders
Product Name:
AMPKa1/AMPKa2 Rabbit Polyclonal Antibody
SKU:
CAB12718
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 13-300 of human PRKAA1/PRKAA2 (NP_006242.5).
The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
Purification Method:
Affinity purification
Gene ID:
5562/5563
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates, using AMPKa1/AMPKa2 Rabbit pAb (CAB12718) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 45s.
