AMPKα1 Polyclonal Antibody (CAB21813)
- SKU:
- CAB21813
- Product Type:
- Antibody
- Antibody Type:
- Polyclonal Antibody
- Reactivity:
- Mouse
- Isotype:
- IgG
- Applications:
- WB
_WB_01__38960.1701341927.jpg?c=2&imbypass=on "Western blot analysis of extracts of Mouse liver, using AMPKα1 antibody (CAB21813) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.")
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Description
| Product Name: | AMPKα1 Polyclonal Antibody |
| SKU: | CAB21813 |
| Size: | 20uL, 100uL |
| Isotype: | IgG |
| Host Species: | IgG |
| Reactivity: | Mouse |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 100-200 of human AMPKα1 (NP_006242.5). |
| Sequence: | IFMV MEYV SGGE LFDY ICKN GRLD EKES RRLF QQIL SGVD YCHR HMVV HRDL KPEN VLLD AHMN AKIA DFGL SNMM SDGE FLRT SCGS PNYA APEV ISGR L |
| Tested Applications: | WB ELISA |
| Recommended Dilution: | WB,1:100 - 1:500 |
| Synonyms: | AMPK; AMPKa1; AMPK alpha 1; AMPKα1 |
| Positive Sample: | Mouse liver |
| Conjugate: | Unconjugated |
| Cellular Localization: | apical plasma membrane, cytoplasm, cytosol, nuclear speck, nucleoplasm, nucleus |
| Calculated MW: | 64kDa |
| Observed MW: | 64kDa |
The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
| Purification Method: | Affinity purification |
| Gene ID: | 5562 |
| Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3. |
 | Western blot analysis of lysates from Mouse liver, using AMPKα1 Rabbit pAb (CAB21813) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s. |
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