The Aminopeptidase N Polyclonal Antibody (CAB5662) is a crucial tool for research involving aminopeptidase N (APN), a cell surface enzyme that plays a key role in various biological processes, including immune regulation and digestion. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By binding specifically to the APN protein, this antibody enables precise detection and analysis in a variety of cell types, making it an ideal choice for studies in immunology, oncology, and gastrointestinal research.
APN, also known as CD13, is a multifunctional enzyme that has been implicated in the regulation of immune responses, angiogenesis, and tumor cell invasion. Its diverse functions make it a valuable target for investigating diseases such as cancer, inflammatory disorders, and gastrointestinal conditions. Understanding the role of APN in these contexts is essential for the development of targeted therapies that can modulate its activity for therapeutic benefit.
Product Name:
CD13/ANPEP Rabbit Polyclonal Antibody
SKU:
CAB5662
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 668-967 of human CD13/CD13/ANPEP (NP_001141.2).
Cell membrane, Cytoplasm, Single-pass type II membrane protein, cytosol.
Calculated MW:
110kDa
Observed MW:
150kDa
Aminopeptidase N is located in the small-intestinal and renal microvillar membrane, and also in other plasma membranes. In the small intestine aminopeptidase N plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. Its function in proximal tubular epithelial cells and other cell types is less clear. The large extracellular carboxyterminal domain contains a pentapeptide consensus sequence characteristic of members of the zinc-binding metalloproteinase superfamily. Sequence comparisons with known enzymes of this class showed that CD13 and aminopeptidase N are identical. The latter enzyme was thought to be involved in the metabolism of regulatory peptides by diverse cell types, including small intestinal and renal tubular epithelial cells, macrophages, granulocytes, and synaptic membranes from the CNS. This membrane-bound zinc metalloprotease is known to serve as a receptor for the HCoV-229E alphacoronavirus as well as other non-human coronaviruses. This gene has also been shown to promote angiogenesis, tumor growth, and metastasis and defects in this gene are associated with various types of leukemia and lymphoma.
Purification Method:
Affinity purification
Gene ID:
290
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates using CD13/ANPEP Rabbit pAb (CAB5662) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
