The Aminoacylase-1 Polyclonal Antibody (CAB6351) is a valuable tool for researchers studying Aminoacylase-1, an enzyme involved in the hydrolysis of aminoacyl compounds. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to Aminoacylase-1, this antibody allows for the detection and analysis of the enzyme in various cell types, making it an excellent choice for research in biochemistry and molecular biology.Aminoacylase-1 plays a crucial role in the metabolism of amino acids and has been implicated in several human diseases, including cancer and metabolic disorders.
Understanding the function and regulation of Aminoacylase-1 is essential for developing targeted therapies for these conditions. The Aminoacylase-1 Polyclonal Antibody provides researchers with a powerful tool for investigating the role of this enzyme in health and disease, advancing our knowledge of amino acid metabolism and its implications for human health.
Product Name:
ACY1 Rabbit Polyclonal Antibody
SKU:
CAB6351
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-408 of human ACY1 (NP_001185824.1).
This gene encodes a cytosolic, homodimeric, zinc-binding enzyme that catalyzes the hydrolysis of acylated L-amino acids to L-amino acids and an acyl group, and has been postulated to function in the catabolism and salvage of acylated amino acids. This gene is located on chromosome 3p21.1, a region reduced to homozygosity in small-cell lung cancer (SCLC), and its expression has been reported to be reduced or undetectable in SCLC cell lines and tumors. The amino acid sequence of human aminoacylase-1 is highly homologous to the porcine counterpart, and this enzyme is the first member of a new family of zinc-binding enzymes. Mutations in this gene cause aminoacylase-1 deficiency, a metabolic disorder characterized by central nervous system defects and increased urinary excretion of N-acetylated amino acids. Alternative splicing of this gene results in multiple transcript variants. Read-through transcription also exists between this gene and the upstream ABHD14A (abhydrolase domain containing 14A) gene, as represented in GeneID:100526760. A related pseudogene has been identified on chromosome 18.
Purification Method:
Affinity purification
Gene ID:
95
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using ACY1 antibody (CAB6351) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
