Anti-AMIGO2 Antibody (PACO37286)

Antibody Name:	AMIGO2 Antibody (PACO37286)
Antibody SKU:	PACO37286
Size:	50ug
Host Species:	Rabbit
Tested Applications:	ELISA, WB, IHC, IF
Recommended Dilutions:	ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:500-1:1000, IF:1:200-1:500
Species Reactivity:	Human, Rat
Immunogen:	Recombinant Human Amphoterin-induced protein 2 protein (420-522AA)

Form:	Liquid
Storage Buffer:	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Purification Method:	>95%, Protein G purified
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

	Western Blot. Positive WB detected in: Rat liver tissue. All lanes: AMIGO2 antibody at 3.2µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 58 kDa. Observed band size: 58 kDa.
	IHC image of PACO37286 diluted at 1:800 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
	IHC image of PACO37286 diluted at 1:800 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Background:	Required for depolarization-dependent survival of cultured cerebellar granule neurons. May mediate homophilic as well as heterophilic cell-cell interaction with AMIGO1 or AMIGO3. May contribute to signal transduction through its intracellular domain. May be required for tumorigenesis of a subset of gastric adenocarcinomas.
Synonyms:	Amphoterin-induced protein 2 (AMIGO-2) (Alivin-1) (Differentially expressed in gastric adenocarcinomas) (DEGA), AMIGO2, ALI1

UniProt Protein Function:	Required for depolarization-dependent survival of cultured cerebellar granule neurons. May mediate homophilic as well as heterophilic cell-cell interaction with AMIGO1 or AMIGO3. May contribute to signal transduction through its intracellular domain. May be required for tumorigenesis of a subset of gastric adenocarcinomas.
UniProt Protein Details:
NCBI Summary:
UniProt Code:	Q86SJ2
NCBI GenInfo Identifier:	219521831
NCBI Gene ID:	347902
NCBI Accession:	NP_001137140.1
UniProt Secondary Accession:	Q86SJ2,Q4VBP6, Q7Z4A0, Q96CN8
UniProt Related Accession:	Q86SJ2
Molecular Weight:	57
NCBI Full Name:	amphoterin-induced protein 2
NCBI Synonym Full Names:	adhesion molecule with Ig like domain 2
NCBI Official Symbol:	AMIGO2
NCBI Official Synonym Symbols:	ALI1; DEGA; AMIGO-2
NCBI Protein Information:	amphoterin-induced protein 2
UniProt Protein Name:	Amphoterin-induced protein 2
UniProt Synonym Protein Names:	AMIGO-2; Alivin-1; Differentially expressed in gastric adenocarcinomas; DEGA
Protein Family:
UniProt Gene Name:	AMIGO2
UniProt Entry Name:

Antibodies

Secondary Antibody