The Amigo2 Polyclonal Antibody (PAC015422) is a valuable tool for researchers studying the Amigo2 protein, which is known to play a role in various cellular processes, such as cell adhesion and migration. This antibody is raised in rabbits and has been validated for use in Western blot applications, allowing for the detection and analysis of Amigo2 in a variety of cell types.Amigo2, also referred to as Alivin-2, is a cell adhesion molecule that has been implicated in neurological development and may have implications in diseases such as cancer and neurodegenerative disorders.
By targeting Amigo2 with this antibody, researchers can gain insight into its functions and potentially develop new therapeutic strategies for related conditions.With its high reactivity towards human samples and its specificity for Amigo2, this polyclonal antibody is an essential tool for studies in cell biology, neuroscience, and cancer research. By utilizing this antibody, researchers can further advance our understanding of Amigo2 and its potential implications in various diseases.
The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using PACO15422(AMIGO2 Antibody) at dilution 1/50, on the right is treated with fusion protein. (Original magnification: x200).
Gel: 6%SDS-PAGE, Lysate: 40 μg, Lane: A172 cells, Primary antibody: PACO15422(AMIGO2 Antibody) at dilution 1/597, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 seconds.
The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using PACO15422(AMIGO2 Antibody) at dilution 1/50, on the right is treated with fusion protein. (Original magnification: x200).
Background:
The amphoterin-induced gene and ORF (AMIGO) family of proteins consists of AMIGO-1, AMIGO-2 and AMIGO-3. All three members are single pass type I membrane proteins that contain several leucine-rich repeats, one IgG domain, and a transmembrane domain. The AMIGO proteins are specifically expressed on fiber tracts of neuronal tissues and participate in their formation. The AMIGO proteins can form complexes with each other, but can also bind itself. AMIGO-1, also designated Alivin-2, promotes growth and fasciculation of neurites and plays a role in myelination and fasciculation of developing neural axons. In cerebellar neurons, AMIGO-2 (Alivin-1) is crucial for depolarization-dependent survival. Similar to AMIGO-1 and AMIGO-2, AMIGO-3 (Alivin-3) plays a role in homophilic and/or heterophilic cell-cell interaction and signal transduction
Synonyms:
adhesion molecule with Ig-like domain 2
UniProt Protein Function:
Required for depolarization-dependent survival of cultured cerebellar granule neurons. May mediate homophilic as well as heterophilic cell-cell interaction with AMIGO1 or AMIGO3. May contribute to signal transduction through its intracellular domain. May be required for tumorigenesis of a subset of gastric adenocarcinomas.
