The Ambra1 Polyclonal Antibody (CAB12578) is a versatile tool for researchers studying the role of Ambra1 in cell biology and autophagy. Ambra1, a key regulator of autophagy and cell survival, is essential for maintaining cellular homeostasis and responding to stressors. This polyclonal antibody, generated in rabbits, is highly specific for Ambra1 and has been validated for use in Western blot and immunofluorescence applications.Ambra1 is known for its involvement in the autophagic process, a cellular mechanism that clears damaged organelles and proteins to maintain cell health. Dysregulation of autophagy has been linked to various diseases, including neurodegenerative disorders and cancer.
By targeting Ambra1 with this antibody, researchers can investigate the molecular mechanisms underlying autophagy and its implications in disease pathology.The Ambra1 Polyclonal Antibody is a valuable tool for studying the function of Ambra1 in various cell types and experimental settings. Its high specificity and sensitivity make it suitable for detecting changes in Ambra1 expression levels, localization, and interactions with other proteins. By using this antibody, researchers can gain deeper insights into the role of Ambra1 in autophagy and its potential therapeutic implications.
Product Name:
Ambra1 Rabbit Polyclonal Antibody
SKU:
CAB12578
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 961-1208 of human Ambra1 (NP_060219.2).
Enables GTPase binding activity and ubiquitin protein ligase binding activity. Involved in macroautophagy; positive regulation of phosphatidylinositol 3-kinase activity; and response to mitochondrial depolarisation. Located in cytosol. Colocalizes with mitochondrion. Biomarker of multiple system atrophy.
Purification Method:
Affinity purification
Gene ID:
55626
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of mouse brain, using Ambra1 Antibody (CAB12578) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25μg per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (AbGn00021)._Exposure time: 90s.
