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ALX3 Colorimetric Cell-Based ELISA (CBCAB00959)

SKU:
CBCAB00959
Product Type:
ELISA Kit
ELISA Type:
Cell Based
Research Area:
Epigenetics and Nuclear Signaling
Reactivity:
Human
Mouse
Rat
Detection Method:
Colorimetric
€769

Description

system_update_altDatasheet

ALX3 Colorimetric Cell-Based ELISA

The ALX3 Colorimetric Cell-Based ELISA kit is a powerful tool designed for the accurate detection of ALX3 levels in cell lysates and tissue samples. This kit offers high sensitivity and specificity, allowing for reliable and reproducible results in a variety of research applications.ALX3 is a key transcription factor that plays a critical role in embryonic development and cell differentiation. Dysregulation of ALX3 has been implicated in various diseases and disorders, making it a valuable biomarker for investigating these conditions and identifying potential therapeutic targets.

With its user-friendly protocol and high-quality components, the ALX3 Colorimetric Cell-Based ELISA kit is an essential resource for researchers looking to study the function and expression of ALX3 in their experimental models. Upgrade your research today with this cutting-edge ELISA kit from AssayGenie.

Product Name:ALX3 Colorimetric Cell-Based ELISA
Product Code:CBCAB00959
ELISA Type:Cell-Based
Target:ALX3
Reactivity:Human, Mouse, Rat
Dynamic Range:> 5000 Cells
Detection Method:Colorimetric 450 nmStorage/Stability:4°C/6 Months
Format:96-Well Microplate

The ALX3 Colorimetric Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can detect ALX3 protein expression profile in cells. The kit can be used for measuring the relative amounts of ALX3 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie siRNA or chemicals), or activators have on ALX3.

Qualitative determination of ALX3 concentration is achieved by an indirect ELISA format. In essence, ALX3 is captured by ALX3-specific primary antibodies while the HRP-conjugated secondary antibodies bind the Fc region of the primary antibody. Through this binding, the HRP enzyme conjugated to the secondary antibody can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of the Cell-Based ELISA, multiple normalization methods are needed:

1. A monoclonal antibody specific for human GAPDH is included to serve as an internal positive control in normalizing the target absorbance values.
2. Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method may be used to determine cell density. After staining, the results can be analysed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.
Database Information:Gene ID: 257, UniProt ID: O95076, OMIM: 606014, Unigene: Hs.669953
Gene Symbol:ALX3
Sub Type:None
UniProt Protein Function:ALX3: Transcriptional regulator with a possible role in patterning of mesoderm during development. Defects in ALX3 are the cause of frontonasal dysplasia type 1 (FND1); also called frontonasal malformation (FNM) or frontorhiny. The term frontonasal dysplasia describes an array of abnormalities affecting the eyes, forehead and nose and linked to midfacial dysraphia. The clinical picture is highly variable. Major findings include true ocular hypertelorism; broadening of the nasal root; median facial cleft affecting the nose and/or upper lip and palate; unilateral or bilateral clefting of the alae nasi; lack of formation of the nasal tip; anterior cranium bifidum occultum; a V-shaped or widow's peak frontal hairline. Belongs to the paired homeobox family.
UniProt Protein Details:

Protein type:DNA-binding; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 1p13.3

Cellular Component: nucleus

Molecular Function:sequence-specific DNA binding

Biological Process: regulation of apoptosis; embryonic forelimb morphogenesis; transcription, DNA-dependent; regulation of transcription, DNA-dependent; embryonic hindlimb morphogenesis; pattern specification process; embryonic skeletal morphogenesis

Disease: Frontonasal Dysplasia 1

NCBI Summary:This gene encodes a nuclear protein with a homeobox DNA-binding domain that functions as a transcriptional regulator involved in cell-type differentiation and development. Preferential methylation of this gene's promoter is associated with advanced-stage neuroblastoma tumors. [provided by RefSeq, Jul 2008]
UniProt Code:O95076
NCBI GenInfo Identifier:113204604
NCBI Gene ID:257
NCBI Accession:NP_006483.2
UniProt Related Accession:O95076
Molecular Weight:
NCBI Full Name:homeobox protein aristaless-like 3
NCBI Synonym Full Names:ALX homeobox 3
NCBI Official Symbol:ALX3  
NCBI Official Synonym Symbols:FND; FND1  
NCBI Protein Information:homeobox protein aristaless-like 3
UniProt Protein Name:Homeobox protein aristaless-like 3
UniProt Synonym Protein Names:Proline-rich transcription factor ALX3
Protein Family:Homeobox protein
UniProt Gene Name:ALX3  
UniProt Entry Name:ALX3_HUMAN
ComponentQuantity
96-Well Cell Culture Clear-Bottom Microplate2 plates
10X TBS24 mL
Quenching Buffer24 mL
Blocking Buffer50 mL
15X Wash Buffer50 mL
Primary Antibody Diluent12 mL
100x Anti-Phospho Target Antibody 60 µL
100x Anti-Target Antibody60 µL
Anti-GAPDH Antibody60 µL
HRP-Conjugated Anti-Rabbit IgG Antibody12 mL
HRP-Conjugated Anti-Mouse IgG Antibody12 mL
SDS Solution12 mL
Stop Solution24 mL
Ready-to-Use Substrate12 mL
Crystal Violet Solution12 mL
Adhesive Plate Seals2 seals

The following materials and/or equipment are NOT provided in this kit but are necessary to successfully conduct the experiment:

  • Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
  • Micropipettes with capability of measuring volumes ranging from 1 µL to 1 ml
  • 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
  • Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
  • Graph paper or computer software capable of generating or displaying logarithmic functions
  • Absorbent papers or vacuum aspirator
  • Test tubes or microfuge tubes capable of storing ≥1 ml
  • Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)
  • Orbital shaker (optional)
  • Deionized or sterile water

*Note: Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.

StepProcedure
1.Seed 200 µL of 20,000 adherent cells in culture medium in each well of a 96-well plate. The plates included in the kit are sterile and treated for cell culture. For suspension cells and loosely attached cells, coat the plates with 100 µL of 10 µg/ml Poly-L-Lysine (not included) to each well of a 96-well plate for 30 minutes at 37°C prior to adding cells.
2.Incubate the cells for overnight at 37°C, 5% CO2.
3.Treat the cells as desired.
4.Remove the cell culture medium and rinse with 200 µL of 1x TBS, twice.
5.Fix the cells by incubating with 100 µL of Fixing Solution for 20 minutes at room temperature. The 4% formaldehyde is used for adherent cells and 8% formaldehyde is used for suspension cells and loosely attached cells.
6.Remove the Fixing Solution and wash the plate 3 times with 200 µL 1x Wash Buffer for five minutes each time with gentle shaking on the orbital shaker. The plate can be stored at 4°C for a week.
7.Add 100 µL of Quenching Buffer and incubate for 20 minutes at room temperature.
8.Wash the plate 3 times with 1x Wash Buffer for 5 minutes each time.
9.Add 200 µL of Blocking Buffer and incubate for 1 hour at room temperature.
10. Wash 3 times with 200 µL of 1x Wash Buffer for 5 minutes each time.
11.Add 50 µL of 1x primary antibodies (Anti-ALX3 Antibody and/or Anti-GAPDH Antibody) to the corresponding wells, cover with Parafilm and incubate for 16 hours (overnight) at 4°C. If the target expression is known to be high, incubate for 2 hours at room temperature.
12.Wash 3 times with 200 µL of 1x Wash Buffer for 5 minutes each time.
13.Add 50 µL of 1x secondary antibodies (HRP-Conjugated AntiRabbit IgG Antibody or HRP-Conjugated Anti-Mouse IgG Antibody) to corresponding wells and incubate for 1.5 hours at room temperature.
14.Wash 3 times with 200 µL of 1x Wash Buffer for 5 minutes each time.
15.Add 50 µL of Ready-to-Use Substrate to each well and incubate for 30 minutes at room temperature in the dark.
16.Add 50 µL of Stop Solution to each well and read OD at 450 nm immediately using the microplate reader.

(Additional Crystal Violet staining may be performed if desired – details of this may be found in the kit technical manual.)

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