The ALS2CR12 Antibody (PAC057404) is a polyclonal antibody designed for research involving ALS2CR12, a protein associated with neurological disorders and neurodegeneration. This antibody is generated in rabbits and has high reactivity with human samples, making it suitable for Western blot applications. By binding to the ALS2CR12 protein, this antibody enables researchers to detect and analyze ALS2CR12 expression in various cell types, making it an ideal tool for studies in neurobiology and neurodegenerative diseases. ALS2CR12, also known as Alsin Rho Guanine Nucleotide Exchange Factor 12, is a key player in regulating neuronal function and survival, making it a potential target for investigating conditions like amyotrophic lateral sclerosis (ALS) and other neurodegenerative disorders.
Understanding the role of ALS2CR12 in the nervous system is crucial for developing therapies to treat these debilitating conditions. The ALS2CR12 Antibody provides researchers with a valuable tool for unraveling the molecular mechanisms underlying neurodegeneration and identifying potential therapeutic targets.
Western Blot. Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate, PC-3 whole cell lysate. All lanes: ALS2CR12 antibody at 12µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 53, 50 kDa. Observed band size: 53 kDa.
Immunofluorescence staining of A549 cells with PACO57404 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
