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Anti-ALPL Antibody (CAB12396)

SKU:
CAB12396
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Signal Transduction
  • Signal Transduction Antibodies 1 Anti-ALPL Antibody CAB12396
  • Signal Transduction Antibodies 1 Anti-ALPL Antibody CAB12396
€139 - €419
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Description

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Antibody Name:Anti-ALPL Antibody
Antibody SKU:CAB12396
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 150-250 of human ALPL (NP_000469.3).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HepG2, U-87MG
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 150-250 of human ALPL (NP_000469.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:ILRW AKDA GKSV GIVT TTRV NHAT PSAA YAHS ADRD WYSD NEMP PEAL SQGC KDIA YQLM HNIR DIDV IMGG GRKY MYPK NKTD VEYE SDEK ARGT RLDG L
Gene ID:249
Uniprot:P05186
Cellular Location:Cell membrane, GPI-anchor, Lipid-anchor
Calculated MW:48kDa/51kDa/57kDa
Observed MW:80kDa
Synonyms:ALPL, AP-TNAP, APTNAP, HOPS, TNAP
Background:This gene encodes a member of the alkaline phosphatase family of proteins. There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature enzyme. This enzyme may play a role in bone mineralization. Mutations in this gene have been linked to hypophosphatasia, a disorder that is characterized by hypercalcemia and skeletal defects.
UniProt Protein Function:ALPL: This isozyme may play a role in skeletal mineralization. Defects in ALPL are a cause of hypophosphatasia (HOPS). HOPS is an inherited metabolic bone disease characterized by defective skeletal mineralization. Four hypophosphatasia forms are distinguished, depending on the age of onset: perinatal, infantile, childhood and adult type. The perinatal form is the most severe and is almost always fatal. Patients with only premature loss of deciduous teeth, but with no bone disease are regarded as having odontohypophosphatasia (odonto). Defects in ALPL are a cause of hypophosphatasia childhood type (HOPSC). Defects in ALPL are a cause of hypophosphatasia infantile type (HOPSI). Belongs to the alkaline phosphatase family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:EC 3.1.3.1; Cofactor and Vitamin Metabolism - folate biosynthesis; Membrane protein, GPI anchor; Motility/polarity/chemotaxis; Phosphatase (non-protein)

Chromosomal Location of Human Ortholog: 1p36.12

Cellular Component: extracellular matrix; extracellular space; membrane; integral to membrane; plasma membrane

Molecular Function:protein binding; pyrophosphatase activity; alkaline phosphatase activity; metal ion binding

Biological Process: osteoblast differentiation; response to antibiotic; response to vitamin D; dephosphorylation; response to glucocorticoid stimulus; reproductive developmental process; response to lipopolysaccharide; skeletal development; endochondral ossification

Disease: Hypophosphatasia, Infantile; Hypophosphatasia, Adult; Hypophosphatasia, Childhood

NCBI Summary:There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. The exact physiological function of the alkaline phosphatases is not known. A proposed function of this form of the enzyme is matrix mineralization; however, mice that lack a functional form of this enzyme show normal skeletal development. This enzyme has been linked directly to hypophosphatasia, a disorder that is characterized by hypercalcemia and includes skeletal defects. The character of this disorder can vary, however, depending on the specific mutation since this determines age of onset and severity of symptoms. Alternatively spliced transcript variants have been described. [provided by RefSeq, Apr 2010]
UniProt Code:P05186
NCBI GenInfo Identifier:68067533
NCBI Gene ID:249
NCBI Accession:P05186.4
UniProt Secondary Accession:P05186,O75090, Q2TAI7, Q59EJ7, Q5BKZ5, Q5VTG5, Q6NZI8 Q8WU32, A1A4E7, B2RMP8, B7Z387, B7Z4Y6,
UniProt Related Accession:P05186
Molecular Weight:51,045 Da
NCBI Full Name:Alkaline phosphatase, tissue-nonspecific isozyme
NCBI Synonym Full Names:alkaline phosphatase, liver/bone/kidney
NCBI Official Symbol:ALPL  
NCBI Official Synonym Symbols:HOPS; TNAP; APTNAP; TNSALP; AP-TNAP  
NCBI Protein Information:alkaline phosphatase, tissue-nonspecific isozyme; glycerophosphatase; tissue-nonspecific ALP; alkaline phosphomonoesterase; liver/bone/kidney-type alkaline phosphatase; alkaline phosphatase liver/bone/kidney isozyme
UniProt Protein Name:Alkaline phosphatase, tissue-nonspecific isozyme
UniProt Synonym Protein Names:Alkaline phosphatase liver/bone/kidney isozyme
Protein Family:Alkaline phosphatase
UniProt Gene Name:ALPL  
UniProt Entry Name:PPBT_HUMAN
Anti-ALPL Antibody (CAB12396)Western blot analysis of extracts of various cell lines, using ALPL antibody (CAB12396) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Anti-ALPL Antibody (CAB12396)Immunohistochemistry of paraffin-embedded mouse kidney using ALPL Rabbit pAb (CAB12396) at dilution of 1:200 (40x lens).

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