The ALPI Polyclonal Antibody (CAB6226) is a valuable tool for researchers studying ALPI, also known as intestinal alkaline phosphatase, an enzyme involved in various physiological processes within the intestines. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications.ALPI plays a vital role in maintaining intestinal health and has been implicated in conditions such as inflammatory bowel disease and intestinal ischemia. By specifically binding to the ALPI protein, this antibody enables accurate detection and analysis in different cell types, making it an ideal choice for studies focusing on gastrointestinal health and disease.
Given the importance of ALPI in intestinal function and its potential as a therapeutic target for gastrointestinal disorders, researchers can benefit greatly from utilizing the ALPI Polyclonal Antibody in their research. This antibody provides a reliable tool for investigating the role of ALPI in health and disease, ultimately contributing to the development of novel treatment strategies in the field of gastroenterology.
Product Name:
ALPI Rabbit Polyclonal Antibody
SKU:
CAB6226
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 20-200 of human ALPI (NP_001622.2).
There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The intestinal alkaline phosphatase gene encodes a digestive brush-border enzyme. This enzyme is a component of the gut mucosal defense system and is thought to function in the detoxification of lipopolysaccharide, and in the prevention of bacterial translocation in the gut.
Purification Method:
Affinity purification
Gene ID:
248
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type (WT) and 293F cells transfected with ALPI using ALPI Rabbit pAb (CAB6226) at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10 s.
