The Alpha2 Adaptin (AP2A2) Monoclonal Antibody is a powerful tool for researchers studying intracellular trafficking and protein transport mechanisms. The antibody, produced through monoclonal techniques, is highly specific for the alpha2 adaptin protein and is validated for use in a variety of applications, including immunofluorescence and flow cytometry.The alpha2 adaptin protein is a key component of the AP2 complex, which plays a crucial role in clathrin-mediated endocytosis and vesicle formation. By targeting the alpha2 adaptin protein, this antibody allows for the visualization and analysis of AP2 complex activity in cells, providing valuable insights into cellular trafficking pathways.
With its high specificity and versatility in applications, the Alpha2 Adaptin (AP2A2) Monoclonal Antibody is an essential tool for researchers investigating intracellular transport processes and seeking to better understand the mechanisms underlying cellular function. Its use can lead to significant advancements in the fields of cell biology, molecular biology, and drug discovery.
Product Name:
Alpha2-adaptin/AP2A2 Monoclonal Antibody
SKU:
CAB21978
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 590-939 of human Alpha2-adaptin/AP2A2 (NP_036437.1).
The protein encoded by this gene is a subunit of the AP-2 adaptor protein complex, which is involved in linking lipid and protein membrane components with the clathrin lattice. This interaction supports the formation of clathrin-coated vesicles, and the encoded subunit aids in the process by binding polyphosphoinositide-containing lipids in the cell membrane.
Purification Method:
Affinity purification
Gene ID:
161
Clone Number:
ARC54447
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using Alpha2-adaptin/AP2A2 antibody (CAB21978) at1:20000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
