The Alpha Mannosidase 2 Polyclonal Antibody (CAB10227) is a valuable tool for researchers studying alpha mannosidase 2, an enzyme involved in the processing of glycoproteins. This antibody, raised in rabbits, has high specificity and sensitivity for human samples, and has been validated for use in Western blot applications. By binding to the alpha mannosidase 2 protein, this antibody enables the detection and analysis of this enzyme in a variety of cell types, making it ideal for studies in glycobiology and cellular biology.
Alpha mannosidase 2 plays a crucial role in the modification and processing of glycoproteins, which are essential for various cellular functions. Dysregulation of alpha mannosidase 2 has been implicated in diseases such as lysosomal storage disorders and cancer, making it a potential target for therapeutic interventions. Understanding the function and regulation of alpha mannosidase 2 is key to advancing research in these areas and developing targeted treatments for associated disorders.
Product Name:
MAN2A1 Rabbit Polyclonal Antibody
SKU:
CAB10227
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 865-1144 of human MAN2A1 (NP_002363.2).
Golgi apparatus membrane, Single-pass type II membrane protein.
Calculated MW:
131kDa
Observed MW:
131-170kDa
This gene encodes a glycosyl hydrolase that localizes to the Golgi and catalyzes the final hydrolytic step in the asparagine-linked oligosaccharide (N-glycan) maturation pathway. Mutations in the mouse homolog of this gene have been shown to cause a systemic autoimmune disease similar to human systemic lupus erythematosus.
Purification Method:
Affinity purification
Gene ID:
4124
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using MAN2A1 antibody (CAB10227) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
