Alpha-Ketoglutarate Fluorometric Assay Kit
- SKU:
- MAES0258
- Product Type:
- Assay
- Instrument:
- Fluorescence Microplate Reader
- Detection Method:
- Fluorometric
- Sample Type:
- Serum, Plasma, Urine, Animal Tissue And Cell Sample
- Sensitivity:
- 0.60 μmol/L
- Range:
- 0.60-100 μmol/L
- Assay Time:
- 35 min
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Description
Product Name: | Alpha-Ketoglutarate Fluorometric Assay Kit |
Product Code: | MAES0258 |
Sample Type: | Serum, Plasma, Urine, Animal Tissue And Cell Sample |
Assay Time: | 35 min |
Assay Type: | Quantitative |
Synonyms: | α-KG |
Detection Instrument: | Fluorescence microplate reader (Ex/Em=535 nm/587 nm) |
Detection Method: | Fluorescence method |
Sensitivity: | 0.60 μmol/L |
Detection Range: | 0.60-100 μmol/L |
Inter-assay CV: | 0.04 |
Intra-assay CV: | 0.02 |
α-Ketoglutaric acid and alanine can be combined with fluorescent probe products under the action of a series of enzymes. The content of α-ketoglutaric acid in samples can be determined by measuring the fluorescence value.
Storage: | -20°C |
Valid Period: | 12 months |
Other Reagents Required: | Normal saline (0.9% NaCl) |
Other Instruments Required: | Incubator, Centrifuge, Fluorescence microplate reader (Ex/Em=535 nm/587 nm), 50 KD ultrafiltration tube |