The Alpha-Fetoprotein Polyclonal Antibody (CAB11013) is a vital tool for research involving alpha-fetoprotein (AFP), a protein that is typically produced by the liver and yolk sac during fetal development, and is also commonly used as a biomarker in cancer diagnostics. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in various applications, including Western blotting. By specifically binding to the AFP protein, researchers can reliably detect and analyze AFP expression in different cell types, making it an essential tool for studies in oncology, developmental biology, and diagnostics.
AFP is known for its significance in liver cancer and certain fetal abnormalities, making it a valuable target for research into early detection and treatment of these conditions. Understanding the role of AFP in different disease states is crucial for developing effective diagnostic and therapeutic strategies.Overall, the Alpha-Fetoprotein Polyclonal Antibody (CAB11013) offers researchers a reliable and specific tool for studying AFP expression and its implications in cancer and developmental disorders, paving the way for advancements in diagnosis and treatment in these critical areas of research.
This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatocarcinoma and with teratoma, and has prognostic value for managing advanced gastric cancer. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly.
Purification Method:
Affinity purification
Gene ID:
174
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of extracts of mouse liver, using Alpha-Fetoprotein (Alpha-Fetoprotein (AFP)) antibody (CAB11013) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 60s.
