The Alpha-Fetoprotein Polyclonal Antibody (CAB0200) is a vital tool for researchers studying Alpha-Fetoprotein (AFP), a protein associated with fetal development and liver cancer. This antibody, produced through immunization of rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By specifically binding to the AFP protein, this antibody enables accurate detection and analysis in a variety of cell types, making it an essential component for investigations in oncology and developmental biology.
Alpha-Fetoprotein is a key biomarker in liver cancer diagnosis and monitoring, as well as in prenatal screening for fetal abnormalities. Its expression in various tissues and its role in cancer progression make AFP a significant target for research in both clinical and basic science settings. Understanding the mechanisms behind AFP regulation and function is crucial for the development of effective diagnostic tools and targeted therapies for liver cancer and other AFP-related conditions.
Recombinant fusion protein containing a sequence corresponding to amino acids 360-609 of human Alpha-Fetoprotein (Alpha-Fetoprotein (AFP)) (NP_001125.1).
This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatocarcinoma and with teratoma, and has prognostic value for managing advanced gastric cancer. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly.
Purification Method:
Affinity purification
Gene ID:
174
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates, using Alpha-Fetoprotein (AFP) Rabbit pAb (CAB0200) at 1:400 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
