The Alpha-Actinin-2 Polyclonal Antibody (CAB3718) is a valuable tool for researchers studying Alpha-Actinin-2, a key cytoskeletal protein involved in cell adhesion and migration. This antibody, produced in rabbits, demonstrates high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to Alpha-Actinin-2, this antibody enables precise detection and analysis of the protein in various cell types, making it an ideal choice for studies in cell biology and cancer research.Alpha-Actinin-2 plays a crucial role in maintaining cell structure and function, particularly in processes like cell movement and signaling.
Dysregulation of Alpha-Actinin-2 has been implicated in various diseases, including cancer and cardiovascular disorders, highlighting its importance as a potential therapeutic target. By investigating the functions of Alpha-Actinin-2 using this antibody, researchers can gain valuable insights into its role in normal cellular processes and disease pathology, paving the way for the development of targeted treatments and interventions.
Product Name:
ACTN2 Rabbit Polyclonal Antibody
SKU:
CAB3718
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human ACTN2 (NP_001094.1).
Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a muscle-specific, alpha actinin isoform that is expressed in both skeletal and cardiac muscles. Several transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
88
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using ACTN2 antibody (CAB3718) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
